Tag: Mycobacterium tuberculosis

Laboratory diagnosis, treatment and prevention of Tuberculosis 4.43/5 (28)

Laboratory diagnosis, treatment and prevention of Tuberculosis

Laboratory diagnosis of Tuberculosis caused by Mycobacterium tuberculosis Specimen and processing sputum, bronchial washings, brushings or biopsies or early morning gastric aspirates, Cerebospinal Fluid (CSF), urine Specimens from sputum and other nonsterile sites should be liquefied with N-acetyl-L-cysteine decontaminated with NaOH (kills many other bacteria and fungi), neutralized with buffer, and concentrated by centrifugation. Specimens […]

Pathogenesis and Clinical manifestation of Mycobacterium tuberculosis 4.5/5 (20)

Pathogenesis and Clinical manifestation of Mycobacterium tuberculosis

Virulence factors of Mycobacterium tuberculosis A. Mycolic acid and Lipoarabinomannan (LAM) Waxy layer that protects the bacteria from many host factors and also to many antibiotics including beta lactamases. Muramyl dipeptide (from peptidoglycan) complexed with mycolic acids can cause granuloma formation, phospholipids induce caseous necrosis. LAM is structurally and functionally related to the O antigenic […]

Habitat and Morphology of Mycobacterium tuberculosis 4.48/5 (27)

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis Habitat Human is only the know reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, facultative or opportunistic pathogens or free living. Usually infect the mono-nuclear phagocytes. Can also be infected by the consumption of unpasteurized milk. […]

Biochemical Characteristics of Mycobacterium tuberculosis 4.8/5 (5)

Biochemical Characteristics of Mycobacterium tuberculosis

Biochemical Characteristics of Mycobacterium tuberculosis S.N. Biochemical Test Results 1 5% NaCl Tolerance Negative (-ve) 2 68°C Catalase Test Negative (-ve) 3 Acid Phosphatase Negative (-ve) 4 Amidase Test Positive (+ve) 5 Arylsulphatase Test Negative (-ve) 6 Growth on P-Nitrobenzoic Acid Negative (-ve) 7 Growth on TCH (10mg/ml) Positive (+ve) 8 Iron Uptake Negative (-ve) […]

Cultural Characteristics of Mycobacterium tuberculosis 4.67/5 (18)

Cultural Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis Culture is the gold standard for laboratory confirmation of Tuberculosis. Growing bacteria are required to perform drug-susceptibility testing and genotyping. In Lowenstein-Jensen (LJ) medium It is an egg based medium and growth is quite slow. It takes 6-8 weeks to get visual colonies on this type of media. Colonies are […]

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